Baylor University Medical Center Proceedings April 2017 - 171

Nitrous oxide-induced vitamin B12 deficiency
Lindsey Stockton, MD, Cameron Simonsen, MD, and Susan Seago, MD

Nitrous oxide is a gas that is odorless, colorless, and has a sweet taste
at room temperature. Nitrous oxide has several uses, including in surgery
and dentistry (referred to as "laughing gas"), in automotive racing, and
in aerosol spray propellants. The aerosol spray propellants that typically
use nitrous oxide are whipped cream canisters and cooking sprays.
Unfortunately, these over-the-counter household items are a source of
nitrous oxide that can be used for recreational use. The most popular is
the use of industrial-grade canisters having the slang term "whippets."
The nitrous oxide can be extracted by pushing the nozzle down slightly
to the side and catching the released gas with a balloon. The contents
of the balloon can then be directly inhaled, giving an instant feeling of
euphoria. This is not a benign means to achieve a euphoric state but can
cause severe nitrous oxide-induced B12 deficiency, which is presented
in this case report.

n estimated 800,000 young adults abuse inhalants every
year (1), and almost 11% of high school seniors report
using inhalants at least once in their lifetime (2). Heavy
inhalant abuse can result in a variety of side eﬀects, including cardiac arrhythmias, hypoxia, metabolic acidosis, and
neurologic deﬁcits. Of all inhalants, nitrous oxide is particularly
toxic due to its conversion of the active monovalent form of
vitamin B12 to its inactive bivalent form (3). We present a case
of subacute combined degeneration of the spinal cord due to
nitrous oxide-induced vitamin B12 deﬁciency.
CASE DESCRIPTION
A 22-year-old man with a history of polysubstance abuse
and asthma presented with a 1-month history of worsening
hand numbness and diﬃculty with ﬁne motor movements,
followed by intermittent numbness in both feet and signiﬁcant gait ataxia. He admitted to daily nitrous oxide abuse for
months prior, reportedly inhaling over 30 whipped cream
chargers every day. Neurologic exam was remarkable for
decreased vibratory sensation in bilateral lower extremities,
absence of distal proprioception, hyperreﬂexia, impaired coordination, rapid alternating movements, truncal ataxia, and
a positive Romberg test. Serologic workup illustrated a mild
anemia with a hemoglobin of 11.7 g/dL and mean corpuscular volume of 98 fL. His vitamin B12 level was at the low
Proc (Bayl Univ Med Cent) 2017;30(2):171-172

Figure. MRI of the cervical spine illustrating a long segment of T2 hyperintensity
involving the dorsal column of virtually the entire cervical spinal cord consistent
with a diagnosis of subacute combined degeneration.

end of normal at 222 pg/mL; however, his homocysteine and
methylmalonic acid levels were elevated at 16.3 μmol/L and
1.56 μmol/L, respectively. Magnetic resonance imaging (MRI)
of the spine illustrated abnormal T2 hyperintensity of the dorsal columns of C1 to T5, consistent with extensive subacute
combined degeneration (Figure). B12 supplementation was
started along with cessation of nitrous oxide use, resulting in
signiﬁcant clinical improvement. The patient was not tested
From the Department of Internal Medicine, Texas A&M Health Science Center
College of Medicine and Baylor Scott and White Memorial Hospital, Temple, Texas.
Corresponding author: Lindsey Stockton, MD, Department of Internal Medicine,
Baylor Scott and White Memorial Hospital, 2401 S. 31st Street, Temple, TX 76508
(e-mail: Lindsey.Stockton@BSWHealth.org).
171

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